Imactiv-3D officially joins the Centre Pierre Potier business incubator
After more than 4 years spent in the Centre Pierre Potier hosted by the ITAV CNRS, Imactiv-3D officially joins the business incubator in the same building.
The 5,000m business incubator is dedicated to the establishment of start-up oriented towards biotechnologies and is located in the campus “Santé du futur” next to the Oncopole in Toulouse (France).
New strategic alliance between Imactiv-3D and NeoVirTech
We have the pleasure to announce a new strategic alliance between Imactiv-3D and NeoVirTech.
NeoVirTech has developed a breakthrough technology to directly visualize and quantify in real time virus/viral vector infection and replication in living cells, from high resolution to high content imaging and screening techniques. The company has been elected in the Top10 drug discovery company in Europe in 2019 (PharmaTechOutlook).
This alliance aims to develop a unique platform with both expertise and innovative tools to move virology projects forward with robust data. Our combined clients will not have to do any compromise on scientific excellence.
NeoVirTech will bring its knowledge and know-how in virology, high content screening imaging and its ANCHOR technology to label viruses in living cells, while Imactiv-3D will bring its expertise in non-invasive 3D imaging (light sheet fluorescence microscopy), image processing and 3D cell culture. Over the past years, we have worked together on several projects, leading to scientific papers.
NeoVirTech and Imactiv-3D are now able to perform:
Preclinical evaluation of compounds efficacy on innovative 3D models by monitoring viral infection and replication using state-of-the-art live imaging techniques. A collection of around 30 viruses is available.
3D quantification and visualization of infection (spheroids, organoids, tissues, organs…) combined with staining of a marker of interest. 3D reconstruction for marketing and communication purposes.
“We are delighted to strengthen our partnership with Imactiv-3D. Their expertise and knowledge in the generation of 3D models for imaging and screening application, combined with our expertise in the visualization of infection and replication using autofluorescent viruses will provide unprecedented models for the understanding of virus propagation in complex environments. This new model will have a deep impact in antiviral screening and oncolytic virotherapies validation for exemple.”Franck Gallardo, Ph.D CEO NeoVirTech SAS “We are very proud to offer our respective customers the best of both our companies. The stability of Neovirtech's Anchor fluorescent marking technology will allow us to make the most of our expertise in 3D model biology, 3D imaging and image processing. This strategic alliance is an end-to-end solution for all needs in understanding and evaluating the efficacy of antiviral treatments.”Jean-Michel Lagarde, CEO Imactiv-3D
WEBINAR Microscopie 3D de la cellule à l'organe : Comment visualiser et quantifier ?
Rendez-vous le 30 juin à 15h, avec notre CEO Jean-Michel Lagarde qui animera un webinar sur la « Microscopie 3D de la cellule à l’organe : Comment visualiser et quantifier ? »
Nous répondrons aux questions suivantes :
- Pourquoi imager en 3D ?
- Comment imager en 3D ?
- Comment exploiter les images obtenues en 3D ?
La présentation sera enrichie d’études de cas.
N’hésitez pas à partager cet événement à vos contacts susceptibles d’être intéressés !
Workshop “Mise en lumière des échantillons 3D”
IMACTIV-3D participe au 1er workshop sur la « Mise en lumière des échantillons 3D » organisé par le CRCT (Centre de Recherche de Cancérologie de Toulouse) à la rentrée prochaine (date à définir).
Notre PDG, Jean-Michel Lagarde, est intervenu pendant ce webinar de préparation, pour présenter l’activité de service en traitement d’image qu’Imactiv-3D mettra à disposition pour les participants.
Vous pouvez retrouver cette présentation ainsi que l’ensemble des interventions des partenaires (CELLINK, IRIT, Miltenyi Biotec, PerkinElmer, ZEISS Microscopy) ici
Ph.D. Defense of Aurélie Gomes
On Tuesday, december 17, 2019, Aurélie Gomes successfully defended her thesis entitled "Evaluation of proliferation in tumor spheroids".
Her work, under a CIFRE agreement, is the result of 3 years of intense collaboration between the "Innovation for the study of 3D proliferation - IP3D" team at ITAV - Institut des Technologies Avancées en sciences du Vivant and IMACTIV-3D.
She was particularly interested in the evaluation of the proliferation in the 3D model of tumor spheroids and its regulation, in particular by oxygen. She has implemented experimental approaches to in toto antibody labelling, light sheet imaging and image analysis.
Her work has led to the development of methods and tools of interest in the study of cancer biology and response in anti-tumor pharmacology. Their application shows the importance of physioxia for the study of proliferation and the evaluation of drug response.
We are very happy that Aurélie Gomes continues the adventure with us. She will join IMACTIV-3D as a young doctor in January 2020 to share her expertise with our customers as R&D project manager in biology and imaging.
Start of APELYM 3D project
Apelym 3D is a new joint project between IMACTIV-3D and INSERM U1048 aiming to analyze the restoration of lymphatic function and adipose tissue by 3D imaging in context of lymphedema. Imactiv-3D will work to implement innovative tools for 3D imaging specific of the lymphatic network in adipose tissue and facilitate the characterization of the effectiveness of therapeutic targets in preclinical models of lymphedema. Alongside Imactiv-3D, two teams of INSERM U1048 are involved Florence Tatin (Team 13), coordinator of the project who works on the molecular regulation of lymphangiogenic factors and Philippe Valet (Team 3) who will bring its expertise on adipokines and adipocytes metabolism. While lymphatic capillaries have been well explored in several tissues, less is known about the deeper collecting lymphatic vessels.
Thanks to our complementary expertise, we will be able to explore this lymphatic network on adipose tissues to understand its involvement in lymphedema. Promising targets would be tested as potential actors of the lymphatic network regeneration. This collaborative project is supported by European Fund and Region Occitanie.
Imactiv-3D at Termis workshop on 3D Bioprinting for Cancer
The TERMIS-EU workshop is an event organized by INSERM Nantes and the University of Nantes on the 26th and 27th of August 2019 in Nantes (France).
The theme for the workshop is “3D bioprinting in cancer research”. This workshop is intended for medical students, doctors and researchers on oncology. Different themes will be addressed, including “3D bioprinting of tumor models, 3D models for cancer, 3D bioprinting and cancer microenvironment” and “3D bioprinting for tissue reconstruction”. Over the two days, practical workshops on 3D modeling and bioprinting are organized.
Imactiv-3D’s CEO, Jean-Michel Lagarde will make a pitch on Exploration and 3D quantification by light sheet imaging and image processing.
If you are interested in joining us for this workshop, you can register here: www.workshop-termis.eu
IMACTIV-3D at the AFSSI connexions in Lyon
Meet Imactiv-3D at the 7th edition annual meeting of preclinical research AFSSI Connexions on July 2-3, 2019 in Lyon (France).
Our CEO, Jean-Michel Lagarde, will be there to present Imactiv-3D’s solutions to support your preclinical research program with innovative imaging and image processing tools. We will be pleased to discuss about your projects and potential collaboration opportunities. Please feel free to contact us to schedule a meeting!
AFSSI Connexions will gather around 300 ecosystem stakeholders during two busy and productive days. Benefit from multiple opportunities to find the know-how, skills, and innovations that you need! Tuesday July 2: Thematic round table about: Business development strategies, Marketplace, International, Research Tax Credit, Growth and Competitiveness. Wednesday, July 3: Business convention day and business oriented presentations. Develop your business and relationships with your colleagues, centers of excellence, platforms and sponsors.
AIS Tours : 7th Antibody Industrial Symposium
Imactiv-3D will attend the 7th edition of the Antibody Industrial Symposium (AIS), organized by MabDesign and LabEx MabImprove on June 24-25 2019 in Tours (France). This event is dedicated to Harnessing the Immune System with Therapeutic Antibodies.
During this event, there will be keynote lectures, industrial talks from big pharmaceutical companies and pitch talks of new creative projects, as well as networking and business partnering time.
The topics covered during the congress are:
Immunomodulation via cell membrane antigens
Harnessing the immune system with immunomodulatory therapeutic antibodies
Pitch Session: Immunomodulation with mAbs: new strategies and technologies
Immunomodulation via FC-FcgrammaR interaction
Immunomodulation via soluble antigens
Jean-Michel Lagarde, our CEO, will represent Imactiv-3D, do not hesitate to get in touch to organize a face to face meeting!
Imactiv-3D at Meet2Win Oncology Partnering Convention
Meet Imactiv-3D at the Oncology Partnering Convention Meet2Win organized on May 21 and 22, 2019 in Bordeaux (France).
MATWIN organizes the 5th edition of this European Partnering Convention dedicated to open innovation, collaborative research and technology transfer.
MEET2WIN is a business convention combining flash project presentations, conferences, one-to-one business meetings as well as exhibition area.
Jean-Michel Lagarde, our CEO, will be there to discuss about the solutions that Imactiv-3D can offer to oncology research stakeholders.
IMALC Project Results
The IMALC project comes to an end! This research project was aiming to treat follicular lymphomas with anti-CD20 antibodies, and to develop quantitative imaging tools to evaluate their efficacy by light sheet microscopy.
To conclude, this transversal and interdisciplinary research project made it possible to:
- Characterize biologically relevant study models
- Model lightsheet imaging clinical observations, and maybe in the longer run, to explain why monoclonal antibodies treatments are less effective in patients with high-tumor burden follicular lymphomas or with an increased immune checkpoint expression.
This project is therefore part of a global research effort to better understand lymphomatous pathology and the mechanisms involved in therapeutic treatments in order to identify targets and anticipate resistance mechanisms. This represents a major challenge for a pathology that is currently considered incurable.
Imactiv-3D team has developed a quantitative analysis of the distribution and diffusion of MAb (Monoclonal antibody) on these 3D models of follicular lymphomas (MALC) based on 1) light sheet microscopy imaging of MALC after treatment with therapeutic ACMs and 2) the development of a custom methodology for efficient and automated image processing and analysis to quantitatively characterize the spatiotemporal biodistribution of MAbs within MALC. This project was based on the skills and expertise found in team N°9 of the CRCT (Toulouse Cancer Research Centre), on the imaging platform of the ITAV (Institut des Technologies Avancées du Vivant) and in the company IMACTIV3D.
Joint publication with INSERM in OncoImmunology
Imactiv-3D is very pleased to announce the publication of a new scientific article in the OncoImmunology Journal on follicular lymphoma, resulting from a collaboration with the Unit UMR1037 of the INSERM in Toulouse (France).
The article, entitled “Boosting γδ T cell-mediated antibody-dependent cellular cytotoxicity by PD-1 blockade in follicular lymphoma” is already available (DOI: 10.1080/2162402X.2018.1554175). This article presents data providing a rational treatment for follicular lymphoma by PD-1 blockade aimed at boosting γδ T cell anti-tumor functions in this pathology.
Imactiv-3D took charge of the image processing of 3D co-cultured MALC for characterization and quantification of the penetration of γδ T cells and monoclonal antibody (GA101).
New scientific publication in virology
Imactiv-3D is pleased to announce the publication of a new scientific article entitled “In vivo labelling of adenovirus DNA identifies chromatin anchoring and biphasic genome replication” in the Journal of Virology (DOI: 10.1128/JVI.00795-18) available here.
This study has been conducted in collaboration with the CNRS UMR5234 lab in Bordeaux (France) and with NeoVirTech. It shows that the real-time genome imaging system developed revealed hitherto uncharacterized features of adenoviral genomes in vivo. This system is capable of identifying new spatiotemporal aspects of the adenovirus life cycle and is potentially transferable to other viral systems with a double-stranded DNA phase.
Imactiv-3D team was involved in the characterization of viral replication centers in human cells and the image processing (denoising and deconvolution).
Imactiv-3D to attend the AFSSI Connexions
Imactiv-3D will attend the 6th edition of the AFSSI Connexions, an annual meeting for preclinical research, on July 2-3 2018 in Marseille (France).
Come and meet Jean-Michel Lagarde, our CEO, to enjoy the 2 days of congress, business convention, exposition and presentations organized by the first French R&D center in life sciences!
The AFSSI Connexions program is:
2nd of July: Panel discussions on the following themes: International, Role of competitiveness clusters, logistic, and customer cooperation
3rd of July: Business convention day, workshop and presentations: Commercial and partnership relations with your peers, clusters, platform etc
Imactiv-3D Sponsor of the 2nd Workshop BioFabrication & Cancer
The « Canceropole Grand Sud-Ouest » is organizing the 2nd edition of the « Biofabrication & Cancer » workshop on 27 and 28 June 2018 in Bordeaux. This edition will be an opportunity to address a wide scientific audience via 6 sessions on a variety of topics and a strong involvement of industrialists in the bio-impression, modelling and cell culture field (pitchs and demonstrations).
Imactiv-3D is a sponsor of this event that will focus on 3D models for clinical practice the first day, and on bio-inks and technologies and 3D models for cancer the second day. Our CEO, Jean-Michel Lagarde will present a pitch explaining the solutions thant Imactiv-3D can offer.
Meet Imactiv-3D at the 4th edition of the Oncology Business Convention Meet2Win organized by MATWIN in Bordeaux (France) on May 17th and 18th 2018.
MATWIN is a French platform supporting innovation in Oncology, subsidiary of Unicancer group (gathering the French Comprehensive Cancer Centers). Meet2Win is a partnering convention fully dedicated to open innovation in Oncology.
This event reunites more than 300 european representatives involved in oncology innovation (pharma, biotechs, startups, investors, researchers, cliniciants, TTOs etc). Jean-Michel Lagarde, our CEO, will be there to talk about our offer for oncology innovation.
IMLINFO (EFA281/16): Start of the European project
IMLINFO is a cross-border network of personalized immunotherapies against non-Hodgkin's lymphoma (NHL) involving 8 organizations located in Spain and France. Its main objective is to determine the efficacy of new immunotherapy treatments by creating a repository of non-Hodgkin's lymphoma (NHL) tumours associated with a three-dimensional (3D) culture platform.
Imactiv-3D at the SF2IC Seminar
Imactiv-3D will attend the 11th edition of the SF2IC Congress organized by the francophone society of engineering and skin imaging (SFIIC) in Marrakech (Morocco) the 22nd and 23th October 2017.
The theme of this event is the bioengineering and objectification of cosmetic effectiveness and it will be addressed during several sessions:
1st day : 2 sessions about pollution and skin, and bio-engineering of cosmetic effectiveness
2nd day : 3 sessions about mechanical tension, tissue engineering, and imaging.
Our CEO Jean-Michel Lagarde, will intervene during the 4th session on the 24th October about tissue engineering. He will speak about the imaging of 3D models of skin structures, from adipocyte to reconstructed skin.
Imactiv-3D at the INEXO Symposium
Our team will be present at the InExO Symposium in Montpellier on 7 July 2017. This event gathers scientists interested in alternative models in vitro, ex ovo and organisms for pathology and ageing research fields.
The development of these new models is a real challenge to respond to societal concerns through an ethical approach aimed at reducing, optimising and replacing (3Rs: reduce, refine, replace) the use of animals for scientific purposes.
Our CEO, Jean-Michel Lagarde and our Ph.D. student Aurélie Gomes are going to present a poster on a new image analysis-based approach to evaluate cytoxicity on 3D models.
Our procedure using quantitative image analysis allows the evaluation and quantification of anticancer drugs cytotoxic activity on 3D multicellular models, and can be applied to multi-parametric analysis to address customers’ specific needs.
Start of IMALC Project on Follicular Lymphomas
The project aims to develop quantitative imaging tools for the evaluation of treatment efficacy of follicular lymphomas using light sheet microscopy. To identify a treatment and evaluate it as objectively as possible requires methods capable of extracting, quantifying and processing information relating to numerous markers. Coupling light sheet microscopy with quantitative image analysis methods directly addresses this need.
This project is co-financed by the ERDF and the Occitanie Region. Imactiv-3D works with two labs: ITAV (CNRS USR3505) and CRCT (INSERM U1037).
The IMALC project aims to carry out an analysis of quantitative distribution and diffusion of monoclonal antibodies (mAbs) on 3D models of lymphomas, based on light sheet microscopy imaging of MALC after treatment with therapeutic monoclonal antibodies and the development of a custom high-performance methodology, automated image processing and analysis to characterize quantitatively the spatiotemporal biodistribution of mAbs within MALCs. Image: Visualization of a MALC using light sheet microscopy 24h-post treatment with GA101. The color gradient (from blue to white) shows the localization of the GA101 intensity.
Imactiv-3D first scientific publication
Imactiv-3D is very proud to announce its first scientific publication in the Oncology Letters!
This scientific article, entitled "Evaluation by quantitative image analysis of anticancer drug activity on multicellular spheroids grown in 3D matrix" is available here (DOI: 10.3892/ol.2016.5221).
Our team developed a new procedure to visualize, analyze and quantify the pharmacological activity of drugs on 3D multicellular spheres grown in a physiological matrix.
The aim of this study was to propose a robust and efficient imaging and analysis pipeline to assess with quantitative parameters the efficacy of a particular cytotoxic drug. HCT116 colorectal adenocarcinoma tumor cell multispheres were observed with structured illumination 3D microscopy and the images obtained were processed with custom analysis tools.
We were thus capable of automatically detecting spheres in a large volume of matrix in 96-well plates. This procedure was used to evaluate drug efficacy in HCT116 spheroids treated with different concentrations of topotecan, a DNA topoisomerase inhibitor. This work demonstrates that quantitative image analysis is an effective mean to evaluate and quantify the cytostatic and cytotoxic activities of anticancer drugs on 3D multicellular models grown in a physiological matrix.
Establishment of the company
Hosting of the company within the CNRS in Toulouse (National Centre for Scientific Research).