Working with Imactiv-3D will improve your preclinical studies

Our expertise in 3D imaging, image processing and cell biology

Imaging

Our unique know-how on a large range of microscopes to get the best out of your biological samples, regardless of their size (sub-cellular, cellular, tissue up to 1 cm3).

  • Expert use of microscopes
    • Wide-field
    • Fluorescence
    • Two-photon
    • Confocal
    • Structured light
    • Live videomicroscopy
    • Light-sheet imaging
  • Consultancy for imaging strategy & modality
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To fully exploit the capabilities of 3D in vitro or ex vivo models, it is essential to preserve the 3D regionalization level of analysis. In addition to confocal and two-photon, we developed a specific expertise in light sheet fluorescence microscopy (LSFM), a fast 3D imaging technique. Once the biological sample prepared, a sheet of laser light passes through it illuminating only a thin slice. A camera placed perpendicularly to the light sheet picks up the fluorescence signal, resulting in images of the illuminated region. This method has many advantages: in addition to being able to image a large range of fixed, live or cleared samples at a great resolution and depth, it also reduces acquisition duration and photodamage.

Image Processing

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Custom-made algorithms based on state-of-the-art image processing tools and in-house innovation will help you with automating manual processes, improving robustness of your results, reinforcing the reproducibility of your experiments and eventually communicating more efficiently.

  • Pre-Processing
    • Denoising
    • Deblurring
    • Image restoration
  • Quantification
    • Segmentation
    • Classification
    • Tailor-made algorithms
    • Deep learning approaches
  • Visualization
    • Surface and volume rendering
    • 3D animation (with customized scenario)
Reliable data and robust results are compulsory to get the most out of images and make a decision with high confidence. Understanding the underlying mechanism of image formation together with mastering the associated mathematics concepts allows us to decipher the blending of efficient and unwanted signals. Image processing is a great way to achieve this goal, with the major advantage of enabling the automatization of your measurements, making the whole image analysis faster and more trustworthy.

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Don't hesitate to contact our team

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Sample Preparation

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Imactiv-3D brings expertise together to optimize the processing of your biological samples in order to achieve outstanding quality imaging.

  • For wide-field microscopy
    • Histological sections, cryosections
    • Large range of labelling (dyes, antibodies, coloration)
  • For 3D imaging
    • Sample clearing and inclusion
    • In vitro 3D models (spheroids, organoids, bioprinting, RHE…)
    • Ex vivo samples (biopsy, tissue, whole organ)
    • Immuno-labelling in toto
It’s no secret that the success of any experiments depends on the quality of the starting material, and this is especially true for imaging biological samples. Sample preparation is crucial to obtain high quality, reproducible and reliable images. That’s why our team has developed innovative protocols to prepare biological samples by labelling and clearing for 3D and wide-field imaging. As every sample is different in terms of size, shape, state and physical properties, we work out the best conditions required for highest quality imaging.

Biological Models

Our team of biologists implements cell biology strategy to study your compounds with better in vivo relevance.

  • Proliferation & viability analysis
    • IC50 on 3D and 2D samples (cytostaticity, cytotoxicity)
    • Cell cycle analysis
  • 3D cell models
    • Free or matrix-embedded spheroids
    • Other on demand
    • Large range of cell lines
    • Cell lines co-culture
  • Controlled-atmosphere culture
    • Hypoxia
    • Physioxia
Imactiv-3D can also process biological samples coming from your labs.
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In vitro 3D models that mimic normal or pathological human tissues are essential for understanding the intimate biological mechanisms and for the development of new treatments.

The interest of 3D models is to accurately reproduce the organization of a tissue, recapitulating the cell-cell connections, cell-microenvironment and cellular heterogeneity found in the micro-regions of a tumor or organ. They are attractive models to predictively assess the anti-proliferative activity of new drugs or genotoxic properties.

With their biological relevance and their cost-saving production, in vitro 3D models are a great way to reduce the number of laboratory animals needed, making the research more ethical.

For more Information

Don't hesitate to contact our team

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