Preclinical trials of a compound efficacy on
skin structure
Our Solutions:
Characterize 3D tissue organization over time
Probes or in toto immunofluorescence
labeling
Light sheet fluorescence microscopy
Quantification and multiparametric analysis
General Procedure
Prior to sample collection by Imactiv-3D:
Sample generation and formalin fixation
Tissue processing:
Possibility to label the sample by probes or in toto immunofluorescence
Sample clearing
Image acquisition:
Light sheet microscopy, from 1X to 20X magnification
Image processing and analysis:
Characterization of cell structure after segmentation: computation of parameters of interest, such as the number of nuclei, the presence of fibers, their morphology, their orientation, the layer thicknessā¦
3D visualization with surface and volume rendering
Application example: visualization and monitoring of reconstructed epidermis differentiation.
Aim:3D visualization of reconstructed epidermis and monitoring of morphological changes in nucleus shape during differentiation.
Tissue labeling:
Propidium iodide (red) to monitor nuclei.
Phalloidin (green) to highlight cortical actin within skin cells.
Images acquired using light sheet fluorescence microscopy.