3D imaging of reconstructed skin model

Your Needs:
  • Qualify 3D models
  • Preclinical trials of a compound efficacy on skin structure

Our Solutions:
  • Characterize 3D tissue organization over time
  • Probes or in toto immunofluorescence labeling
  • Light sheet fluorescence microscopy
  • Quantification and multiparametric analysis

General Procedure

Prior to sample collection by Imactiv-3D:
  • Sample generation and formalin fixation

Tissue processing:
  • Possibility to label the sample by probes or in toto immunofluorescence
  • Sample clearing

Image acquisition:
  • Light sheet microscopy, from 1X to 20X magnification

Image processing and analysis:
  • Characterization of cell structure after segmentation: computation of parameters of interest, such as the number of nuclei, the presence of fibers, their morphology, their orientation, the layer thicknessā€¦
  • 3D visualization with surface and volume rendering

Application example: visualization and monitoring of reconstructed epidermis differentiation.

  • Aim:3D visualization of reconstructed epidermis and monitoring of morphological changes in nucleus shape during differentiation.
  • Tissue labeling:
    • Propidium iodide (red) to monitor nuclei.
    • Phalloidin (green) to highlight cortical actin within skin cells.

  • Images acquired using light sheet fluorescence microscopy.
  • Nuclei volume reconstructed in 3D (right figure).