Preclinical trials of a compound efficacy on
Characterize 3D tissue organization over time
Probes or in toto immunofluorescence
Light sheet fluorescence microscopy
Quantification and multiparametric analysis
Prior to sample collection by Imactiv-3D:
Sample generation and formalin fixation
Possibility to label the sample by probes or in toto immunofluorescence
Light sheet microscopy, from 1X to 20X magnification
Image processing and analysis:
Characterization of cell structure after segmentation: computation of parameters of interest, such as the number of nuclei, the presence of fibers, their morphology, their orientation, the layer thickness…
3D visualization with surface and volume rendering
Application example: visualization and monitoring of reconstructed epidermis differentiation.
Aim:3D visualization of reconstructed epidermis and monitoring of morphological changes in nucleus shape during differentiation.
Propidium iodide (red) to monitor nuclei.
Phalloidin (green) to highlight cortical actin within skin cells.
Images acquired using light sheet fluorescence microscopy.