3D ex vivo imaging and characterization of vascular network in rodent
Study of the impact of pathologies on the cardiac vascular network
Preclinical study of treatment efficacy
Light sheet microscopy and clearing to characterize the vascular network in 3D
Automated 3D image processing for microvascular network quantification
Prior to sample collection by Imactiv-3D:
In vivo labelling by infusion with a fluorescent lectin before euthanasia.
Formalin fixation of extracted sample
3D light sheet fluorescence microscopy
Image processing and analysis:
Quantitative characterization of the vascular network
Extraction of efficient volume
Computation of parameters of interest: vessels length and local size, density of the vascular network
3D visualization with surface and volume rendering
Reconstruction of the whole sample
Advanced display using 3D animations
Characterization of the microvascular network of a whole rat heart.
Whole sample acquisition using 6 to 9 fields of view
Raw data amounts to about 200 GB or 100 billion voxels
Image processing takes 14 hours using a parallel architecture
Analysis of the microvascular network in 33 samples following this procedure exhibited an internal variation of only 3.5 % with respect to the mean.
Assessment of microvascular alteration in pathological rat with comorbidities such as hypertension/diabetes (and effect of a potential treatment on vascular density).