3D ex vivo imaging and characterization of brain vascular network

Your Needs:
  • Study of the impact of pathologies on the brain micro-vascular network
  • Preclinical study of treatment efficacy

Our Solutions:
  • Light sheet microscopy and clearing to characterize the vascular network in 3D
  • Automated 3D image processing for micro-vascular network quantification

General Procedure

Prior to sample collection by Imactiv-3D:
  • In vivo labelling by infusion with a fluorescent lectin before euthanasia
  • Formalin fixation of extracted sample

Image acquisition:
  • Optical clearing of samples
  • 3D light sheet fluorescence microscopy
  • Multi-position acquisition

Image processing and analysis:
  • Quantitative characterization of the vascular network
    • Vessels segmentation
    • Extraction of efficient volume
    • Computation of parameters of interest: vessels length and local size, density of the vascular network
  • 3D visualization with surface and volume rendering
    • Reconstruction of the whole sample
    • Advanced display using 3D animations

Application example

Study of Alzheimer pathology:

  • Healthy brain versus pathology-induced brain.
  • Diseased brain cortex shows a drastic decrease in vascular network density, together with a modified distribution of the vessels size.
  • Results consistent with the inflammation induction described in the literature.