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Robust imaging and analysis process to assess with quantitative parameters the efficacy of a cytotoxic drug. The image represents an example of a Hoechst
dye in blue for the recognition of cell clusters and an EDU dye in green for the proliferative aspect.
This procedure allows by using quantitative image analysis to evaluate and quantify the cytotoxic activity of anticancer drugs on 3D multicellular models, and can be applied to multi-parametric analysis to address customers’ specific needs.

Evaluation of drugs using 3D pre-clinical in vitro model

CytotoxicityMulti-Spheroid

Robust imaging and analysis process to assess with quantitative parameters the efficacy of a cytotoxic drug. The image represents an example of a Hoechst dye in blue for the recognition of cell clusters and an EDU dye in green for the proliferative aspect. This procedure allows by using quantitative image analysis to evaluate and quantify the cytotoxic activity of anticancer drugs on 3D multicellular models, and can be applied to multi-parametric analysis to address customers’ specific needs.

3D imaging of a double labeling of a spheroid

CytotoxicitySpheroid

3D visualization and reconstruction of a two-photon z-stack of a spheroid of tumor cells grown in a matrix of hyaluronic acid. Nuclei were stained by using DAPI and the actin cytoskeleton stained with phalloidin-alexa488.

Imaging of preadipocyte lipid droplet production

HumanAdipocyteSkinHypodermis

Images made with an apotome videomicroscope. The quality of the images produced allows us to automatically quantify the production of lipid droplets by image processing. Process developed in collaboration with DIVA Expertise.

Imaging of preadipocyte collagene production

HumanAdipocyteSkinHypodermis

Images made with an apotome videomicroscope. Automatic quantification of the production of collagne by image processing. Process developed in collaboration with DIVA Expertise.

Volume rendering of a multicellular spheroid obtained from MCF-7 human breast cancer cell lines with a propidium iodide (PI) staining after 3 days of culture.
Images taken by wide-field light sheet microscopy, with a resolution of 0.645 µm in XY and 1.3 µm in Z. Spheroid diameter is about 250 µm.

Volume rendering of a Spheroid based on light sheet imaging

CytotoxicitySpheroid

Volume rendering of a multicellular spheroid obtained from MCF-7 human breast cancer cell lines with a propidium iodide (PI) staining after 3 days of culture. Images taken by wide-field light sheet microscopy, with a resolution of 0.645 µm in XY and 1.3 µm in Z. Spheroid diameter is about 250 µm.

3D vizualisation of the rat liver vascular network

AnimalsRatLiverVascular Network

Images taken by wide-field light sheet microscopy, after sample transparisation, without any staining. Automatic vascular network recognition by image processing.

3D vizualisation of a mouse lung

AnimalsMouseLung

Images taken by wide-field light sheet microscopy, after sample clearing, without any staining.

3D exploration and quantification for the study of heart disease

AnimalsMouseHeart

Images taken by wide-field light sheet microscopy, after sample transparisation, without any staining. Automatic ventricule recognition by image processing.

3D vizualisation of a mouse eye

AnimalsMouseEye

Images taken by wide-field light sheet microscopy, after sample clearing without any staining.

3D vizualisation of a mouse intestine

AnimalsMouseIntestine

Images taken by wide-field light sheet microscopy, after sample transparisation, without any staining.

3D vizualisation of a mouse kidney

AnimalsMouseKidney

Images taken by wide-field light sheet microscopy, after sample clearing without any staining.

Light-sheet imaging of a rat heart

AnimalsRatHeart

3D reconstruction from 16 acquisitions, resulting from 4 angles of view at 90 ° from each other, with for each angle of view 4 acquisitions made with a vertical overlap of 30%. Each of the 16 volumes includes 900 images of 1500 x 1000 pixels, a total of about 20 billion voxels. The resolution is 7.7 μm in XYZ. The data set is 40 GB. Permanent occlusion by ligation of the left coronary artery was performed to mimic myocardial infarction.

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